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pspax2, pmd2.g plasmid and psin4-based lentiviral vectors  (Addgene inc)


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    Structured Review

    Addgene inc pspax2, pmd2.g plasmid and psin4-based lentiviral vectors
    Pspax2, Pmd2.G Plasmid And Psin4 Based Lentiviral Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psin4-based+lentiviral+vectors/pspax2/pmc09599480-64-25-27
    Average 90 stars, based on 1 article reviews
    pspax2, pmd2.g plasmid and psin4-based lentiviral vectors - by Bioz Stars, 2026-10
    90/100 stars

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    Transfection:

    Article Title: Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-like Cells.
    Article Snippet: .. One day after seeding 293T cells at 3 × 106 cells per 10-cm dish, we transfected the cells with PsPAX2, pMD2.G plasmid and pSIN4-based lentiviral vectors (Addgene, Watertown, MA, USA) using Lipofectamine 3000 (Invitrogen, Waltham, MA, USA). .. The virus-containing supernatants were collected at 48 h and 72 h post-transfection and filtered through 0.45-μm cellulose acetate filters (Millipore, Burlington, MA, USA).

    Plasmid Preparation:

    Article Title: Direct Reprogramming of Mouse Subchondral Bone Osteoblasts into Chondrocyte-like Cells.
    Article Snippet: .. One day after seeding 293T cells at 3 × 106 cells per 10-cm dish, we transfected the cells with PsPAX2, pMD2.G plasmid and pSIN4-based lentiviral vectors (Addgene, Watertown, MA, USA) using Lipofectamine 3000 (Invitrogen, Waltham, MA, USA). .. The virus-containing supernatants were collected at 48 h and 72 h post-transfection and filtered through 0.45-μm cellulose acetate filters (Millipore, Burlington, MA, USA).



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